Assessment of substrate inhibition of bacterial oligopeptidase B.

Crystal Structures of Trypanosoma brucei Oligopeptidase B Broaden the Paradigm of Catalytic Regulation in Prolyl Oligopeptidase Family Enzymes

Oligopeptidase B cleaves after basic amino acids in peptides up to 30 residues. As a virulence factor in bacteria and trypanosomatid pathogens that is absent in higher eukaryotes, this is a promising drug target. Here we present ligand-free open state and inhibitor-bound closed state crystal structures of oligopeptidase B from Trypanosoma brucei, the causative agent of African sleeping sickness...

Assessment of Agaricus Bisporus S-II Extract as a Bio-Controlling Agent against Human Pathogenic Bacterial Species

Agaricus bisporus mushrooms are well known for their nutritional and medicinal values. A. bisporus is a source of protein (about 40% on a dry basis), ergosterol, several minerals, carbohydrate, and fat. The present study was conducted to investigate the effect of A. bisporus S-II extracts on human pathogenic bacteria in-vitro condition. Totally, thre...

Substrate recognition properties of oligopeptidase B from Salmonella enterica serovar Typhimurium.

Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmonella enterica serovar Typhimurium and demonstrate that it exhibits amidolytic ...

Effects of Substrate Solution pH, Incubation Temperature, and Calcination Temperature on Bacterial Synthesis of Nano-Crystalline Hydroxyapatite

Effects of Substrate Solution pH, Incubation Temperature, and Calcination Temperature on Bacterial Synthesis of Nano-Crystalline Hydroxyapatite